Project
Binding

Part:BBa_K1378007:Design

Designed by: Zhang Zijian & Meng Liuyi   Group: iGEM14_Peking   (2014-10-06)

INPNC-MVN & GFP (the Binding Plasmid)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1495
    Illegal NheI site found at 1518
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 466
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2196
    Illegal SapI site found at 1038


Design Notes

We optimized the sequence of MVN beforehand and chose two different promoters to avoid possible homologous recombination. We also designed a 10aa flexible protein domain linker between MVN and INPNC to help the two domains function normally.


Source

This is a composite part so we constructed it using various different smaller parts. INPNC comes from the existing part BBa_K523013. The sequence of MVN can be found in the genomic sequence of Microcystis aeruginosa PCC7806 and the gene was synthesized by a company (sequence can be found in the part BBa_K1378003). GFP is common and comes from the existing part BBa_E0040.

References